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- Table of Contents
PRKAA2 antibody (M01420) performs well in IF, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Fluorescent image of Hela cell stained with PRKAA2 Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.PRKAA2 immunoreactivity is localized to Cytoplasm significantly.
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Anti-PRKAA2 Antibody
PRKAA2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IF, WB |
Recommend Dilution
Application
Dilution
Species
IF: 1:10-1:50
WB: 1:1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti-PRKAA2 Antibody
View all PRKAA2 Antibodies
View all PRKAA2 Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
206CT5.4.2
Isotype
IgG2a,K
Immunogen
This PRKAA2 monoclonal antibody is generated from mouse immunized with PRKAA2 recombinant protein.
Calculated Molecular Weight
62.3 kDa
Background of PRKAA2
The protein encoded by this gene is a catalytic subunit of
the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
consisting of an alpha catalytic subunit, and non-catalytic beta
and gamma subunits. AMPK is an important energy-sensing enzyme that
monitors cellular energy status. In response to cellular metabolic
stresses, AMPK is activated, and thus phosphorylates and
inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
regulating de novo biosynthesis of fatty acid and cholesterol.
Studies of the mouse counterpart suggest that this catalytic
subunit may control whole-body insulin sensitivity and is necessary
for maintaining myocardial energy homeostasis during ischemia.
Cite This Product
Anti-PRKAA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01420)
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Product Promise
We promise Anti-PRKAA2 Antibody performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01420
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026