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Product Info Summary
| SKU: | M01420 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-PRKAA2 Antibody
SKU/Catalog Number
M01420
Size
80 µl
Description
Boster Bio Anti-PRKAA2 Antibody (Catalog # M01420). Tested in IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PRKAA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01420)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
206CT5.4.2
Isotype
IgG2a,K
Immunogen
This PRKAA2 monoclonal antibody is generated from mouse immunized with PRKAA2 recombinant protein.
Reactive Species
M01420 is reactive to PRKAA2 in Human
Calculated molecular weight
62.3 kDa
Background of PRKAA2
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01420 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent image of Hela cell stained with PRKAA2 Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.PRKAA2 immunoreactivity is localized to Cytoplasm significantly.
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All lanes : Anti-PRKAA2 Antibody at 1:1000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Hela whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 62 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-PRKAA2 Antibody (M01420)
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