Click image to see more details
Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IF, IHC, ICC, WB|
Customers Who Bought This Also Bought
Anti-PRMT1 Rabbit Monoclonal Antibody
Boster Bio Anti-PRMT1 Rabbit Monoclonal Antibody catalog # M01417. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRMT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01417)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human PRMT1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01417 is reactive to PRMT1 in Human, Mouse, Rat
M01417 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
Submit A Review
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PRMT1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate (M01417).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT1 monoclonal antibody (Catalog # M01417) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT1
Gene/Protein Information For PRMT1 (Source: Uniprot.Org, NCBI)
Protein arginine N-methyltransferase 1
class I-like SAM-binding methyltransferase superfamily
ANM1; EC 2.1.1; EC 2.1.1.-; EC 18.104.22.168; EC 22.214.171.124; HCP1; Histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae); HMT1; HMT2; HRMT1L2; HRMT1L2HMT1 hnRNP methyltransferase-like 2; Interferon receptor 1-bound protein 4; IR1B4; IR1B4protein arginine N-methyltransferase 1; PRMT1; protein arginine methyltransferase 1 PRMT1 ANM1, HCP1, HRMT1L2, IR1B4 protein arginine methyltransferase 1 protein arginine N-methyltransferase 1|HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2|heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2|histone-arginine N-methyltransferase PRMT1|interferon receptor 1-bound protein 4*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PRMT1, check out the PRMT1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PRMT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M01417
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-PRMT1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-PRMT1 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question