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Product Info Summary
| SKU: | A04242 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Probable hydrolase PNKD PNKD Antibody
SKU/Catalog Number
A04242
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Probable hydrolase PNKD PNKD Antibody catalog # A04242. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Probable hydrolase PNKD PNKD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04242)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human PNKD(NP_056303.3).
Reactive Species
A04242 is reactive to PNKD in Human, Mouse
Calculated molecular weight
42.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04242 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using PNKD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
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Immunofluorescence analysis of U2OS cells using PNKD antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Specific Publications For Anti-Probable hydrolase PNKD PNKD Antibody (A04242)
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