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Product Info Summary
| SKU: | A05975 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Proline-rich protein 5 PRR5 Antibody
SKU/Catalog Number
A05975
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Proline-rich protein 5 PRR5 Antibody (Catalog # A05975). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PRR5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Proline-rich protein 5 PRR5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05975)
Host
Rabbit
Contents
PRR5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PRR5 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PRR5. The immunogen is located within amino acids 310 - 360 of PRR5.
Cross-reactivity
PRR5 antibody is predicted to not cross-react with other Protor protein family members.
Reactive Species
A05975 is reactive to PRR5 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
42.8 kDa
Background of PRR5
Proline-rich protein 5 (PRR5), also known as Protor-1, is a 388 amino acid protein in Protor family, is thought to act as a tumor suppressor in breast and colorectal tumorigenesis. PRR5 is widely expressed and possesses two RICTOR interaction sites and a C-terminal Proline rich region. It promotes Rapamycin complex 2 (mTORC2) activity. There are four isoforms of PRR5 that are produced as a result of alternative splicing events and these isoforms play an important role in the modulation of platelet-derived growth factor signaling and in the regulation of PDGFR-beta expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05975 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PRR5 antibody can be used for detection of PRR5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PRR5 in SK-N-SH cell lysate with PRR5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
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Immunohistochemistry of PPR5 in mouse brain tissue with PPR5 antibody at 5 μg/mL.
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Immunofluorescence of PPR5 in mouse brain tissue with PPR5 antibody at 20 μg/mL.
Specific Publications For Anti-Proline-rich protein 5 PRR5 Antibody (A05975)
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