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Product Info Summary
| SKU: | A09074 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Prolyl 3-hydroxylase OGFOD1 OGFOD1 Antibody
SKU/Catalog Number
A09074
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Prolyl 3-hydroxylase OGFOD1 OGFOD1 Antibody (Catalog # A09074). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
OGFOD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Prolyl 3-hydroxylase OGFOD1 OGFOD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09074)
Host
Rabbit
Contents
OGFOD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
OGFOD1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human OGFOD1. The immunogen is located within the first 50 amino acids of OGFOD1.
Cross-reactivity
OGFOD1 antibody is predicted to not cross-react with other TPA1 protein family members. At least two isoforms are known to exist; this antibody will only detect the larger isform.
Reactive Species
A09074 is reactive to OGFOD1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
63.2 kDa
Background of OGFOD1
OGFOD1, a member of the 2-OG-Fe(II) dioxygenase family, plays an important role in ischemic survival and stress. It is an oxidoreductase containing a Prolyl 4-hydroxylase alpha subunit. An OGFOD1 iron binding residue is required for ATPAF1 gene expression. As a novel stress granule component, OGFOD1 plays important proapoptotic roles in the regulation of translation and HRI-mediated phosphorylation of eIF2alpha in cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09074 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
OGFOD1 antibody can be used for detection of OGFOD1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of OGFOD1 in Daudi cell lysate with OGFOD1 antibody at 1 μg/mL.
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Immunofluorescence of OGFOD1 in Daudi cells with OGFOD1 antibody at 20 μg/mL.
Specific Publications For Anti-Prolyl 3-hydroxylase OGFOD1 OGFOD1 Antibody (A09074)
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