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Product Info Summary
| SKU: | M02082-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Prostatic Acid Phosphatase ACPP Monoclonal Antibody
SKU/Catalog Number
M02082-1
BM5745 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Prostatic Acid Phosphatase ACPP Monoclonal Antibody catalog # M02082-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prostatic Acid Phosphatase ACPP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02082-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFFB-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAP ( prostatic acid phosphatase)
Reactive Species
M02082-1 is reactive to ACPP in Human
Observed Molecular Weight
54 kDa
Calculated molecular weight
44.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02082-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human prostate cancer
Validation Images & Assay Conditions
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Western blot analysis of PAP ( prostatic acid phosphatase) expression in human prostate cancer lysate.
Specific Publications For Anti-Prostatic Acid Phosphatase ACPP Monoclonal Antibody (M02082-1)
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Customer Q&As
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5 Customer Q&As for Anti-Prostatic Acid Phosphatase ACPP Monoclonal Antibody
Question
We are currently using anti-Prostatic Acid Phosphatase Monoclonal antibody M02082-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The anti-Prostatic Acid Phosphatase Monoclonal antibody (M02082-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
Question
Would anti-Prostatic Acid Phosphatase Monoclonal antibody M02082-1 work on horse IF with placenta?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
Our lab technicians have not tested anti-Prostatic Acid Phosphatase Monoclonal antibody M02082-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Prostatic Acid Phosphatase Monoclonal antibody M02082-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse placenta in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-15
Question
Do you have a BSA free version of anti-Prostatic Acid Phosphatase Monoclonal antibody M02082-1 available?
F. Anderson
Verified customer
Asked: 2018-09-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Prostatic Acid Phosphatase Monoclonal antibody M02082-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-17
Question
Would anti-Prostatic Acid Phosphatase Monoclonal antibody M02082-1 work for IF with placenta?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
According to the expression profile of placenta, ACPP is highly expressed in placenta. So, it is likely that anti-Prostatic Acid Phosphatase Monoclonal antibody M02082-1 will work for IF with placenta.
Boster Scientific Support
Answered: 2018-09-04
Question
Will M02082-1 anti-Prostatic Acid Phosphatase Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
It shows on the product datasheet, M02082-1 anti-Prostatic Acid Phosphatase Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-29
