This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Sftpc antibody (A02001) performs well in ELISA, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
Filter:
Western blot analysis of Prosurfactant Protein C/SFTPC using anti-Prosurfactant Protein C/SFTPC antibody (A02001).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prosurfactant Protein C/SFTPC antigen affinity purified polyclonal antibody (Catalog # A02001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prosurfactant Protein C/SFTPC at approximately 21KD. The expected band size for Prosurfactant Protein C/SFTPC is at 21KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prosurfactant Protein C/SFTPC antigen affinity purified polyclonal antibody (Catalog # A02001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prosurfactant Protein C/SFTPC at approximately 21KD. The expected band size for Prosurfactant Protein C/SFTPC is at 21KD.
Bundle Save
Anti-Prosurfactant Protein C/SFTPC Antibody Picoband®
Sftpc antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, WB |
Tested Applications
Sftpc antibody was positively detected by WB in:
rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Prosurfactant Protein C/SFTPC Antibody Picoband®
View all SFTPC Antibodies
View all SFTPC Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Prosurfactant Protein C/SFTPC recombinant protein (Position: S98-I193).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated Molecular Weight
21.1 kDa
Background of Sftpc
SFTPC (Surfactant pulmonary -associated protein C), also known as SFTP2, SPC, PSP-C or PULMONARY SURFACTANT PROTEIN SP5, is a protein that in humans is encoded by the SFTPC gene. It is a membrane protein which manufactures surfactant. Glasser et al. (1988) assigned the SFTPC gene, symbolized SFTP2 by the Human Gene Mapping nomenclature committee, to chromosome 8 by hybridization to somatic cell hybrid DNA. Wood et al. (1994) identified 4 cosmids at the SFTP2 locus by cDNA hybridization. Using a polymorphic CA repeat in the SFTP2 gene and linkage analysis in 8 CEPH families, they located the gene close to D8S298; maximum lod = 19.3 at theta = 0.01. They regionalized the gene to 8p21 by fluorescence in situ hybridization. Moore et al. (1992) showed that the corresponding mouse gene, Sftp2, maps to chromosome 14.
Cite This Product
Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02001)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Sftpc antibody
Publications
Application:
Species:
Neutrophil extracellular traps trigger alveolar epithelial cell necroptosis through the cGAS-STING pathway during acute lung injury in mice
Published: 2024-09-03
Journal: International Journal of Biological Sciences
Application: IF
Impact Factor: 8.20
Species: Mouse
PMID: 39309425
CGRP is essential for protection against alveolar epithelial cell necroptosis by activating the AMPK/L-OPA1 signaling pathway during acute lung injury
Published: 2024-01-09
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 4.50
Species: Mouse
PMID: 38193350
Fabrication of sac-like hydrogel membranes for replicating curved tissue barriers on chips
Published: 2023-11-20
Journal: LAB ON A CHIP
Impact Factor: 6.10
PMID: 38018218
A microfluidic lung-on-a-chip based on biomimetic hydrogel membrane
Published: 2023-05-17
Journal: BIOTECHNOLOGY AND BIOENGINEERING
Application: IF
Impact Factor: 3.80
Species: Human
PMID: 37195718
DNA repair enzyme OGG1 promotes alveolar progenitor cell renewal and relieves PM2.5-induced lung injury and fibrosis
Published: 2020-09-22
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: IF
Impact Factor: 4.87
Species: Human
PMID: 32977282
Review
Have you used Anti-Prosurfactant Protein C/SFTPC Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02001
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026