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Product Info Summary
| SKU: | A02001 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Prosurfactant Protein C/SFTPC Antibody Picoband®
SKU/Catalog Number
A02001
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® catalog # A02001. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02001)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Prosurfactant Protein C/SFTPC recombinant protein (Position: S98-I193).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02001 is reactive to Sftpc in Mouse, Rat
Observed Molecular Weight
21 kDa
Calculated molecular weight
21.1 kDa
Background of Sftpc
SFTPC (Surfactant pulmonary -associated protein C), also known as SFTP2, SPC, PSP-C or PULMONARY SURFACTANT PROTEIN SP5, is a protein that in humans is encoded by the SFTPC gene. It is a membrane protein which manufactures surfactant. Glasser et al. (1988) assigned the SFTPC gene, symbolized SFTP2 by the Human Gene Mapping nomenclature committee, to chromosome 8 by hybridization to somatic cell hybrid DNA. Wood et al. (1994) identified 4 cosmids at the SFTP2 locus by cDNA hybridization. Using a polymorphic CA repeat in the SFTP2 gene and linkage analysis in 8 CEPH families, they located the gene close to D8S298; maximum lod = 19.3 at theta = 0.01. They regionalized the gene to 8p21 by fluorescence in situ hybridization. Moore et al. (1992) showed that the corresponding mouse gene, Sftp2, maps to chromosome 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02001 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat lung tissue, mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of Prosurfactant Protein C/SFTPC using anti-Prosurfactant Protein C/SFTPC antibody (A02001).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prosurfactant Protein C/SFTPC antigen affinity purified polyclonal antibody (Catalog # A02001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prosurfactant Protein C/SFTPC at approximately 21KD. The expected band size for Prosurfactant Protein C/SFTPC is at 21KD.
Specific Publications For Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® (A02001)
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