Anti-Protein AATF AATF Antibody

Product Name

Anti-Protein AATF AATF Antibody

View all AATF Antibodies

SKU/Catalog Number

A03945

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein AATF AATF Antibody (Catalog # A03945). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

AATF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protein AATF AATF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03945)

Host

Rabbit

Contents

AATF Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

AATF antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human AATF. The immunogen is located within the last 50 amino acids of AATF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least four isoforms of RABEX5 are known to exist; this antibody will recognize all four. RABEX5 antibody is predicted to not cross-react with other LRRFIP family members.

Reactive Species

A03945 is reactive to AATF in Human, Mouse

Applications

A03945 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

63.133kDa

Background of AATF

AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases. AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways. Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the beta-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer's disease (AD), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AATF antibody can be used for the detection of AATF by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5

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Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 μg/mL.

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Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 μg/mL.

Gene/Protein Information For AATF (Source: Uniprot.org, NCBI)

Alternative Names

apoptosis antagonizing transcription factor; Apoptosis-antagonizing transcription factor; CHE-1; CHE1Rb-binding protein Che-1; DEDprotein AATF AATF BFR2, CHE-1, CHE1, DED apoptosis antagonizing transcription factor protein AATF|rb-binding protein Che-1

For more info on AATF, check out the AATF Infographic

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In this infographic, you will see the following information for AATF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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