Anti-Phospho-Che-1 (AATF) (Ser477) Antibody

Product Name

Anti-Phospho-Che-1 (AATF) (Ser477) Antibody

View all AATF Antibodies

SKU/Catalog Number

P03945

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Che-1 (AATF) (Ser477) Antibody (Catalog # P03945). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Che-1 (AATF) (Ser477) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03945)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

608

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03945 is reactive to AATF in Human

Applications

P03945 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

63.133kDa

Background of AATF

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser⁴⁷⁷ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Gene/Protein Information For AATF (Source: Uniprot.org, NCBI)

Alternative Names

apoptosis antagonizing transcription factor; Apoptosis-antagonizing transcription factor; CHE-1; CHE1Rb-binding protein Che-1; DEDprotein AATF AATF BFR2, CHE-1, CHE1, DED apoptosis antagonizing transcription factor protein AATF|rb-binding protein Che-1

For more info on AATF, check out the AATF Infographic

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In this infographic, you will see the following information for AATF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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