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Product Info Summary
| SKU: | A02837 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein KIBRA WWC1 Antibody
SKU/Catalog Number
A02837
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein KIBRA WWC1 Antibody (Catalog # A02837). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
WWC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Protein KIBRA WWC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02837)
Host
Rabbit
Contents
WWC1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1. The immunogen is located within amino acids 60 - 110 of WWC1.
Cross-reactivity
WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A02837 is reactive to WWC1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
125.3 kDa
Background of WWC1
The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1). WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3). WWC1 is phosphorylated by the mitotic kinases Aurora-A and –B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02837 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WWC1 in human brain tissue lysate with WWC1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of WWC1 in mouse brain tissue with WWC1 antibody at 5 μg/ml.
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Immunofluorescence of WWC1 in mouse brain tissue with WWC1 antibody at 20 μg/ml.
Specific Publications For Anti-Protein KIBRA WWC1 Antibody (A02837)
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