Product Info Summary
| SKU: | A17432 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein NipSnap homolog 3B NIPSNAP3B Antibody
SKU/Catalog Number
A17432
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein NipSnap homolog 3B NIPSNAP3B Antibody (Catalog # A17432). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
NIPSNAP3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein NipSnap homolog 3B NIPSNAP3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17432)
Host
Rabbit
Contents
NIPSNAP3B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NIPSNAP3B antibody was raised against a 16 amino acid synthetic peptide near the center of human NIPSNAP3B. The immunogen is located within amino acids 40 - 90 of NIPSNAP3B.
Cross-reactivity
NIPSNAP3B antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.
Reactive Species
A17432 is reactive to NIPSNAP3B in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.3 kDa
Background of NIPSNAP3B
The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low. NIPSNAP proteins have been suggested to be important in vesicular transport.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17432 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NIPSNAP3B antibody can be used for detection of NIPSNAP3B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NIPSNAP3B in mouse brain tissue lysate with NIPSNAP3B antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of NIPSNAP3B in mouse brain tissue with NIPSNAP3B antibody at 2.5 μg/mL.
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Immunofluorescence of NIPSNAP3B in mouse brain tissue with NIPSNAP3B antibody at 5 μg/mL.
Specific Publications For Anti-Protein NipSnap homolog 3B NIPSNAP3B Antibody (A17432)
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