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Product Info Summary
| SKU: | A16391 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein shisa-4 SHISA4 Antibody
SKU/Catalog Number
A16391
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein shisa-4 SHISA4 Antibody (Catalog # A16391). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SHISA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Protein shisa-4 SHISA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16391)
Host
Rabbit
Contents
SHISA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
SHISA4 antibody was raised against a 15 amino acid peptide near the center of human SHISA4 . The immunogen is located within amino acids 100 - 150 of SHISA4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SHISA4 antibody is predicted to not cross-react with other SHISA protein family members.
Reactive Species
A16391 is reactive to Shisa4 in Human, Mouse
Applications
A16391 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
21.483kDa
Background of Shisa-4
SHISA4 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SHISA4 Antibody can be used for detection of SHISA4 by Western blot starting at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of SHISA4 in mouse brain tissue with SHISA4 antibody at 2.5 μg/ml.
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Immunofluorescence of SHISA4 in mouse brain tissue with SHISA4 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For Shisa4 (Source: Uniprot.org, NCBI)
Gene Name
Shisa4
Full Name
Protein shisa-4
Weight
21.483kDa
Superfamily
shisa family
Alternative Names
C1orf40; chromosome 1 open reading frame 40; FLJ18233; hShisa4; MGC129812; MGC129813; protein shisa-4; shisa homolog 4 (Xenopus laevis); Shisa4; Shisa-4; TMEM58; Transmembrane protein 58MGC102893
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Shisa4, check out the Shisa4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Shisa4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein shisa-4 SHISA4 Antibody (A16391)
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No publications found for A16391
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Customer Reviews
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