Anti-Phospho-Protein Tyrosine Phosphatase H1 (PTPH1)(Ser459) Antibody

Product Name

Anti-Phospho-Protein Tyrosine Phosphatase H1 (PTPH1)(Ser459) Antibody

View all PTPN3 Antibodies

SKU/Catalog Number

P06227

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Protein Tyrosine Phosphatase H1 (PTPH1)(Ser459) Antibody (Catalog # P06227). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Protein Tyrosine Phosphatase H1 (PTPH1)(Ser459) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06227)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 of human PTPH1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P06227 is reactive to PTPN3 in Human, Mouse

Applications

P06227 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

103.99kDa

Background of PTPN3

Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser-459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser-459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of mouse testicular lysate showing specific immunolabeling of the ~104 kDa PTPH1 phosphorylated at Ser⁴⁵⁹ in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (gamma-Ptase , 400 units/100ul lysate for 30 minutes, RT).

Gene/Protein Information For PTPN3 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686N0569; EC 3.1.3.48; protein tyrosin phosphatase H1; protein tyrosine phosphatase, non-receptor type 3; Protein-tyrosine phosphatase H1; PTP-H1; PTPH1cytoskeletal-associated protein tyrosine phosphatase; tyrosine phosphatase; tyrosine-protein phosphatase non-receptor type 3 PTPN3 PTP-H1, PTPH1 protein tyrosine phosphatase non-receptor type 3 tyrosine-protein phosphatase non-receptor type 3|cytoskeletal-associated protein tyrosine phosphatase|protein-tyrosine phosphatase H1

For more info on PTPN3, check out the PTPN3 Infographic

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In this infographic, you will see the following information for PTPN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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