Anti-Protocadherin-12 PCDH12 Antibody

Product Name

Anti-Protocadherin-12 PCDH12 Antibody

View all PCDH12 Antibodies

SKU/Catalog Number

A10847

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protocadherin-12 PCDH12 Antibody (Catalog # A10847). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PCDH12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protocadherin-12 PCDH12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10847)

Host

Rabbit

Contents

PCDH12 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12. The immunogen is located within amino acids 30 - 80 of PCDH12.

Cross-reactivity

This antibody is predicted to not cross-react with PCDH18.

Reactive Species

A10847 is reactive to PCDH12 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

129.0 kDa

Background of PCDH12

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2, 289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10847 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PCDH12

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