Anti-Protocadherin-18 PCDH18 Antibody

Product Name

Anti-Protocadherin-18 PCDH18 Antibody

View all PCDH18 Antibodies

SKU/Catalog Number

A12047

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protocadherin-18 PCDH18 Antibody (Catalog # A12047). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PCDH18 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protocadherin-18 PCDH18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12047)

Host

Rabbit

Contents

PCDH18 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PCDH18 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH18. The immunogen is located within amino acids 50 - 100 of PCDH18.

Cross-reactivity

At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12.

Reactive Species

A12047 is reactive to PCDH18 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

126.1 kDa

Background of PCDH18

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 18 (PCDH18) was initially identified through screening of human and mouse ESTs using conserved cytoplasmic domain motifs. Tissue screening revealed PCDH18 broad expression in tissues such as brain, liver, heart, kidney, lung, and trachea. PCDH18 expression is also temporally and spatially regulated in the mouse embryonic brain and interacts with Disabled-1, an intracellular adapter protein involved in neuronal migration and cell positioning during mammalian brain development. PCDH18 was also found to be expressed in the developing zebrafish neural tube and central nervous system, lending support to the hypothesis that PCDH18 may play a role during brain development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A12047 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PCDH18 antibody can be used for detection of PCDH18 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PCDH18

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis