Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband®

PROX1 antibody

Boster Bio Anti-Prospero homeobox protein 1 PROX1 Antibody catalog # PA2229. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PA2229
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband®

View all PROX1 Antibodies

SKU/Catalog Number

PA2229
BA2390-2 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Prospero homeobox protein 1 PROX1 Antibody catalog # PA2229. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2229)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PROX1, identical to the related mouse and rat sequences.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2229 is reactive to PROX1 in Human, Mouse, Rat

Observed Molecular Weight

55 kDa

Calculated molecular weight

83.2 kDa

Background of PROX1

Prospero homeobox protein 1, also called PROX1 is a protein that in humans is encoded by the PROX1 gene. This gene is mapped to 1q32.3. The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2229 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Thymus Tissue, HELA Cell, JURKAT Cell, MM231 Cell,

Validation Images & Assay Conditions

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4 Customer Q&As for Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband®

Question

you antibody using your anti-PROX1 antibody for ventricular cardiac muscle tissue morphogenesis studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

Thank you for your inquiry. This PA2229 anti-PROX1 antibody is tested on rat thymus tissue, tissue lysate, hela cell lysate, jurkat cell lysate, mm231 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-23

Question

We have observed staining in human liver. Are there any suggestions? Is anti-PROX1 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-05-06

Answer

From what I have seen in literature liver does express PROX1. From what I have seen in Uniprot.org, PROX1 is expressed in liver, embryonic brain, muscle, among other tissues. Regarding which tissues have PROX1 expression, here are a few articles citing expression in various tissues:
Embryonic brain, Pubmed ID: 8812486
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-06

Question

Our team were happy with the WB result of your anti-PROX1 antibody. However we have observed positive staining in muscle nucleus. using this antibody. Is that expected? Could you tell me where is PROX1 supposed to be expressed?

Z. Jha

Verified customer

Asked: 2018-12-18

Answer

According to literature, muscle does express PROX1. Generally PROX1 expresses in nucleus. Regarding which tissues have PROX1 expression, here are a few articles citing expression in various tissues:
Embryonic brain, Pubmed ID: 8812486
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-12-18

Question

We are currently using anti-PROX1 antibody PA2229 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

S. Carter

Verified customer

Asked: 2014-06-20

Answer

The anti-PROX1 antibody (PA2229) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-06-20

Order DetailsPrice
PA2229

100μg

$370
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100 μg HRP conjugated (liquid)

$570
PA2229-PE

100 μg PE conjugated (liquid)

$820
PA2229-carrier-free

Carrier Free

$370
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PA2229
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 25, 2026