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Product Info Summary
| SKU: | A15727-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-Pre-mRNA-processing factor 39 PRPF39 Antibody
SKU/Catalog Number
A15727-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Pre-mRNA-processing factor 39 PRPF39 Antibody (Catalog# A15727-1). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pre-mRNA-processing factor 39 PRPF39 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15727-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PRPF39. Immunogen sequence location: 263-312
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15727-1 is reactive to PRPF39 in Human, Mouse
Applications
A15727-1 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
78.43kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from K562 cells, using PRPF39 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PRPF39 (Source: Uniprot.org, NCBI)
Gene Name
PRPF39
Full Name
Pre-mRNA-processing factor 39
Weight
78.43kDa
Superfamily
PRP39 family
Alternative Names
FLJ11128; FLJ20666; FLJ45460; MGC149842; MGC149843; pre-mRNA-processing factor 39; PRP39 homolog; PRP39 pre-mRNA processing factor 39 homolog (S. cerevisiae); PRP39 pre-mRNA processing factor 39 homolog (yeast) PRPF39 pre-mRNA processing factor 39 pre-mRNA-processing factor 39|PRP39 homolog|PRP39 pre-mRNA processing factor 39 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRPF39, check out the PRPF39 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRPF39: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pre-mRNA-processing factor 39 PRPF39 Antibody (A15727-1)
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No publications found for A15727-1
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Pre-mRNA-processing factor 39 PRPF39 Antibody
Question
I see that the anti-PRPF39 antibody A15727-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-04
Question
We are currently using anti-PRPF39 antibody A15727-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
The anti-PRPF39 antibody (A15727-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-08
Question
Will A15727-1 anti-PRPF39 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Kulkarni
Verified customer
Asked: 2015-02-05
Answer
As indicated on the product datasheet, A15727-1 anti-PRPF39 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-02-05
