Anti-PRR7 Purified Monoclonal Antibody

Product Name

Anti-PRR7 Purified Monoclonal Antibody

View all PRR7 Antibodies

SKU/Catalog Number

M13414

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PRR7 Purified Monoclonal Antibody (Catalog# M13414). Tested in WB, ICC application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-PRR7 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13414)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

TRAP3/10

Isotype

Mouse IgG2a

Immunogen

Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253). The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M13414 is reactive to PRR7 in Human, Mouse, Rat

Applications

M13414 is guaranteed for ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

30.93kDa

Background of PRR7

PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100.
Western blotting: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer).

Validation Images & Assay Conditions

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Figure 1. Immunocytochemistry staining of PRR7 using Anti-PRR7 Purified Monoclonal Antibody (M13414).

Immunocytochemistry staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).

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Figure 2. Western blotting validation for Anti-PRR7 Purified Monoclonal Antibody M13414

Western blotting detection of PRR7 / TRAP3 in murine brain lysate using the monoclonal antibody TRAP3/10.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For PRR7 (Source: Uniprot.org, NCBI)

Alternative Names

MGC10772; proline rich 7 (synaptic); proline-rich protein 7; Synaptic proline-rich membrane protein PRR7 proline rich 7, synaptic proline-rich protein 7|synaptic proline-rich membrane protein

For more info on PRR7, check out the PRR7 Infographic

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In this infographic, you will see the following information for PRR7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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