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Product Info Summary
| SKU: | M02867 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PSB9 / LMP2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02867
BM5582 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PSB9 / LMP2 Rabbit Monoclonal Antibody catalog # M02867. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year. Avoid repeated freezing and thawing
Cite This Product
Anti-PSB9 / LMP2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02867)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEFO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Proteasome 20S LMP2
Reactive Species
M02867 is reactive to PSMB9 in Human, Mouse, Rat
Observed Molecular Weight
21 kDa
Calculated molecular weight
23.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02867 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human A431 whole cell, human HL-60 whole cell, human Raji whole cell, human HEL whole cell, rat liver tissue, rat spleen tissue, mouse liver tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of PSB9 using anti-PSB9 antibody (M02867).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSB9 antigen affinity purified monoclonal antibody (M02867) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSB9 at approximately 21 kDa. The expected band size for PSB9 is at 21 kDa.
Specific Publications For Anti-PSB9 / LMP2 Rabbit Monoclonal Antibody (M02867)
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Customer Q&As
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4 Customer Q&As for Anti-PSB9 / LMP2 Rabbit Monoclonal Antibody
Question
We are currently using anti-PSB9 / LMP2 Monoclonal antibody M02867 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
R. Wu
Verified customer
Asked: 2020-02-17
Answer
The anti-PSB9 / LMP2 Monoclonal antibody (M02867) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
What sample target on Western Blot application was tested with M02867?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of Proteasome 20S LMP2 expression in A431 cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
We need to test anti-PSB9 / LMP2 Monoclonal antibody M02867 on mouse leukocyte for research purposes, then I may be interested in using anti-PSB9 / LMP2 Monoclonal antibody M02867 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The products we sell, including anti-PSB9 / LMP2 Monoclonal antibody M02867, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-13
Question
See attached the WB image, lot number and protocol we used for leukocyte using anti-PSB9 / LMP2 Monoclonal antibody M02867. Please let me know if you require anything else.
C. Mitchell
Verified customer
Asked: 2016-01-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-01
