Anti-PSMA5 Antibody

Product Name

Anti-PSMA5 Antibody

View all Proteasome 20S alpha 5 Antibodies

SKU/Catalog Number

M07499

Size

80 µl

Description

Boster Bio Anti-PSMA5 Antibody (Catalog # M07499). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PSMA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07499)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

426CT8.5.1

Isotype

IgG1

Immunogen

Purified His-tagged PSMA5 protein (Fragment) was used to produced this monoclonal antibody.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M07499 is reactive to PSMA5 in Human, Mouse, Rat

Applications

M07499 is guaranteed for IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

26.411kDa

Background of Proteasome 20S alpha 5

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:1000
IHC-P: 1:25

Validation Images & Assay Conditions

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Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody. M07499 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

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PSMA5 Antibody (Cat. #M07499) western blot analysis in K562 cell line lysates (35μg/lane). This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).

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All lanes : Anti-PSMA5 Antibody at 1:1000 dilution
Lane 1: human liver lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: Neuro-2a whole cell lysate
Lane 6: PC-12 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa.
Blocking/Dilution buffer: 5% NFDM/TBST.

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Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody (Cat#M07499). M07499 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Gene/Protein Information For PSMA5 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.25.1; FLJ42315; macropain subunit zeta; Macropain zeta chain; MGC117302; MGC125802; MGC125803; MGC125804; Multicatalytic endopeptidase complex zeta chain; proteasome (prosome, macropain) subunit, alpha type, 5; proteasome alpha 5 subunit; proteasome component 5; proteasome subunit alpha type-5; proteasome subunit zeta; Proteasome zeta chain; PSC5; ZETA PSMA5 PSC5, ZETA proteasome 20S subunit alpha 5 proteasome subunit alpha type-5|epididymis tissue sperm binding protein Li 11n|macropain subunit zeta|macropain zeta chain|multicatalytic endopeptidase complex zeta chain|proteasome (prosome, macropain) subunit, alpha type, 5|proteasome alpha 5 subunit|proteasome component 5|proteasome subunit alpha 5|proteasome subunit zeta|proteasome zeta chain

For more info on PSMA5, check out the PSMA5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PSMA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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