PSMA5 antibody (A07499-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
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Anti-PSMA5 Antibody Picoband®

PSMA5 antibody

Reactive SpeciesHostApplication
Human, Monkey, Mouse, RatRabbitELISA, Flow Cytometry, IF, IHC, ICC, WB

SKU: A07499-1

Size: 100 µg

Bundle to save: I do not want free secondary ab
Price:
$370/100 µg

In stock. Ships Next Business Day.

Quantity: 1

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Guarantee / Product Promise

Tested Applications

PSMA5 antibody was positively detected by WB in:

human Hela whole cell, human HepG2 whole cell, human 293T whole cell, monkey COS-7 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse Neuro-2a whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

PSMA5 antibody was positively detected by IHC in:

human liver cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

PSMA5 antibody was positively detected by ICC/IF in:

U2OS cell

PSMA5 antibody was positively detected by FC in:

Hela cell

Recommend Dilution

Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Reactive Data

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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-PSMA5 Antibody Picoband®
View all PSMA5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PSMA5 recombinant protein (Position: M1-I241). Human PSMA5 shares 100% and 98.8% amino acid (aa) sequence identity with mouse and rat PSMA5, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
26 kDa
Calculated Molecular Weight

26.4 kDa

Background of PSMA5
Proteasome subunit alpha type-5 also known as 20S proteasome subunit alpha-5 is a protein that in humans is encoded by the PSMA5 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene.
Cite This Product
Anti-PSMA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07499-1)

Protocols

Western Blot protocol for PSMA5 antibody
Immunohistochemistry (IHC) protocol for PSMA5 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PSMA5 antibody
Flow Cytometry (FC) protocol for PSMA5 antibody

Review

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Product Promise

We promise Anti-PSMA5 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
A07499-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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Boster Guarantee

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 15, 2026