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Product Info Summary
| SKU: | M02188-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PSMB8 / LMP7 Monoclonal Antibody
SKU/Catalog Number
M02188-2
BM5593 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PSMB8 / LMP7 Monoclonal Antibody catalog # M02188-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSMB8 / LMP7 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02188-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEFI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Proteasome 20S LMP7 The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Reactive Species
M02188-2 is reactive to PSMB8 in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
30.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02188-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:100
Validation Images & Assay Conditions
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Western blot analysis of Proteasome 20S LMP7 expression in U937 cell lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Specific Publications For Anti-PSMB8 / LMP7 Monoclonal Antibody (M02188-2)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PSMB8 / LMP7 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02188-2?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Proteasome 20S LMP7 expression in U937 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-PSMB8 / LMP7 Monoclonal antibody M02188-2 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
A. Lewis
Verified customer
Asked: 2018-12-04
Answer
The anti-PSMB8 / LMP7 Monoclonal antibody (M02188-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-04
Question
My question regarding product M02188-2, anti-PSMB8 / LMP7 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Parker
Verified customer
Asked: 2017-05-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02188-2 anti-PSMB8 / LMP7 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-31
Question
Please see the WB image, lot number and protocol we used for liver using anti-PSMB8 / LMP7 Monoclonal antibody M02188-2. Please let me know if you require anything else.
R. Collins
Verified customer
Asked: 2014-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-31
