Anti-PSMC5 Antibody Picoband™

Product Name

Anti-PSMC5 Antibody Picoband™

View all SUG1 Antibodies

SKU/Catalog Number

A05480-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PSMC5 Antibody Picoband™ catalog # A05480-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PSMC5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05480-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PSMC5 recombinant protein (Position: E29-K393).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05480-1 is reactive to PSMC5 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

45-49 kDa

Calculated molecular weight

48608 MW

Background of SUG1

26S protease regulatory subunit 8, also known as 26S proteasome AAA-ATPase subunit Rpt6, is an enzyme that in humans is encoded by the PSMC5 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05480-1 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PSMC5 using anti-PSMC5 antibody (A05480-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CCRF-CEM whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human SK-OV-3 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC5 antigen affinity purified polyclonal antibody (Catalog # A05480-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC5 at approximately 45-49 kDa. The expected band size for PSMC5 is at 45-49 kDa.

Gene/Protein Information For PSMC5 (Source: Uniprot.org, NCBI)

Alternative Names

MSUG1 protein; p45; p45/SUG26S protease regulatory subunit 8; proteasome (prosome, macropain) 26S subunit, ATPase, 5; proteasome 26S ATPase subunit 5; Proteasome 26S subunit ATPase 5; Proteasome subunit p45; S8; SUG-1; SUG126S proteasome AAA-ATPase subunit RPT6; Tat-binding protein homolog 10; TBP10; thyroid receptor interactor 1; TRIP1Thyroid hormone receptor-interacting protein 1 PSMC5 RPT6, S8, SUG-1, SUG1, TBP10, TRIP1, p45, p45/SUG proteasome 26S subunit, ATPase 5 26S proteasome regulatory subunit 8|26S protease regulatory subunit 8|26S proteasome AAA-ATPase subunit RPT6|MSUG1 protein|Tat-binding protein homolog 10|proteasome (prosome, macropain) 26S subunit, ATPase, 5|proteasome 26S ATPase subunit 5|proteasome subunit p45|testicular tissue protein Li 149|thyroid hormone receptor-interacting protein 1|thyroid receptor interactor 1

For more info on PSMC5, check out the PSMC5 Infographic

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In this infographic, you will see the following information for PSMC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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