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Product Info Summary
| SKU: | A09882-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-PSMD13 Antibody Picoband®
SKU/Catalog Number
A09882-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSMD13 Antibody Picoband® catalog # A09882-1. Tested in WB, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PSMD13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09882-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PSMD13 recombinant protein (Position: E68-E364). Human PSMD13 shares 97.3% and 97.6% amino acid (aa) sequence identity with mouse and rat PSMD13, respectively.
Reactive Species
A09882-1 is reactive to PSMD13 in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.9 kDa
Background of PSMD13
26S proteasome non-ATPase regulatory subunit 13 is an enzyme that in humans is encoded by the PSMD13 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09882-1 is guaranteed for ELISA, Flow Cytometry, IP, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human, Mouse, Rat |
| Immunoprecipitation | 0.5-2 μg/ml | Human |
| Flow Cytometry (Fixed) | 1-3 μg/1x106 cells | Human |
| ELISA | 0.1-0.5 μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of PSMD13 using anti-PSMD13 antibody (A09882-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD13 antigen affinity purified polyclonal antibody (Catalog # A09882-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD13 at approximately 43 kDa. The expected band size for PSMD13 is at 43 kDa.
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Flow Cytometry analysis of Hela cells using anti-PSMD13 antibody (A09882-1).
Overlay histogram showing Hela cells stained with A09882-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD13 Antibody (A09882-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Immunoprecipitating (IP) PSMD13 in HepG2 whole cell lysate.
Western blot analysis of PSMD13 using anti-PSMD13 antibody (A09882-1);
Lane 1: HepG2 whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-PSMD13 antibody in HepG2 whole cell lysate;
Lane 3: anti-PSMD13 antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSMD13 antigen affinity purified polyclonal antibody (A09882-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PSMD13 at approximately 43 kDa. The expected band size for PSMD13 is at 43 kDa.
Specific Publications For Anti-PSMD13 Antibody Picoband® (A09882-1)
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