Anti-PSMD2 Antibody Picoband™

Product Name

Anti-PSMD2 Antibody Picoband™

View all Proteasome 19S 10B Antibodies

SKU/Catalog Number

A06642-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PSMD2 Antibody Picoband™ catalog # A06642-3. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PSMD2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06642-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PSMD2 recombinant protein (Position: K219-L908).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06642-3 is reactive to PSMD2 in Human, Monkey, Rat

Applications

A06642-3 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

97 kDa

Calculated molecular weight

100.2kDa

Background of Proteasome 19S 10B

26S proteasome non-ATPase regulatory subunit 2, also as known as 26S Proteasome Regulatory Subunit Rpn1 (systematic nomenclature), is an enzyme that in humans is encoded by the PSMD2 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Monkey, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PSMD2 using anti-PSMD2 antibody (A06642-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human SK-OV-3 whole cell lysates,
Lane 5: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD2 antigen affinity purified polyclonal antibody (Catalog # A06642-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD2 at approximately 97 kDa. The expected band size for PSMD2 is at 97,100 kDa.

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Figure 2. IF analysis of PSMD2 using anti-PSMD2 antibody (A06642-3).
PSMD2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMD2 Antibody (A06642-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PSMD2 (Source: Uniprot.org, NCBI)

Alternative Names

26S proteasome regulatory subunit RPN1; 26S proteasome regulatory subunit S2; MGC14274,55.11 protein; P97; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; Protein 55.11; Rpn1; S226S proteasome non-ATPase regulatory subunit 2; TNFR-associated protein 2; TRAP226S proteasome subunit p97; tumor necrosis factor receptor-associated protein 2; Tumor necrosis factor type 1 receptor-associated protein 2 PSMD2 P97, RPN1, S2, TRAP2 proteasome 26S subunit ubiquitin receptor, non-ATPase 2 26S proteasome non-ATPase regulatory subunit 2|55.11 protein|TNFR-associated protein 2|proteasome (prosome, macropain) 26S subunit, non-ATPase, 2|proteasome 26S subunit, non-ATPase 2|protein 55.11|tumor necrosis factor type 1 receptor-associated protein 2

For more info on PSMD2, check out the PSMD2 Infographic

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In this infographic, you will see the following information for PSMD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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