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Product Info Summary
|Reactive Species:||Cow, Human, Mouse, Rat|
|Application:||Flow Cytometry, IF, IHC, ICC, WB|
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Anti-PTGER2 Monoclonal Antibody
Boster Bio Anti-PTGER2 Monoclonal Antibody catalog # M04963. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Cow, Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTGER2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04963)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human PTGER2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M04963 is reactive to PTGER2 in Cow, Human, Mouse, Rat
M04963 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTGER2 expression in JAR cell lysate using anti-PTGER2 antibody (M04963).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER2 antigen affinity purified polyclonal antibody (Catalog # M04963) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Gene/Protein Information For PTGER2 (Source: Uniprot.Org, NCBI)
Prostaglandin E2 receptor EP2 subtype
G-protein coupled receptor 1 family
EP2; PGE receptor EP2 subtype; PGE2 receptor EP2 subtype; prostaglandin E receptor 2 (subtype EP2), 53kD; prostaglandin E receptor 2 (subtype EP2), 53kDa; prostaglandin E2 receptor EP2 subtype; Prostanoid EP2 receptor; PTGER2 PTGER2 EP2 prostaglandin E receptor 2 prostaglandin E2 receptor EP2 subtype|PGE receptor EP2 subtype|PGE2 receptor EP2 subtype|prostaglandin E receptor 2 (subtype EP2), 53kD|prostaglandin E receptor 2 (subtype EP2), 53kDa|prostanoid EP2 receptor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PTGER2, check out the PTGER2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PTGER2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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