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Product Info Summary
| SKU: | A02481 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-PTPN13/PTPL1 (FAP1) Antibody
SKU/Catalog Number
A02481
Size
50 µl
Form
Liquid
Description
Boster Bio Anti-PTPN13/PTPL1 (FAP1) Antibody (Catalog# A02481). Tested in IP, IHC, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-PTPN13/PTPL1 (FAP1) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02481)
Host
Rabbit
Contents
50 μl sera
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A recombinant protein of human FAP-1 (amino acids 1279 to 1883)
Reactive Species
A02481 is reactive to PTPN13 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
276.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02481 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC (paraffin): 1:1000-1:5000
IHC (frozen): Users should optimize
IP: 1:50-1:200
Validation Images & Assay Conditions
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Immunohistochemisty of FAP-1 in formalin-fixed, paraffin embedded ovarian carcinoma cores from a tissue microarray using primary antibody dilution ratio of 1:2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-eosin counterstain.
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Immunocytochemical analysis of FAP-1 in cell lines using primary antibody dilution ratio of 1:2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively. The staining data correlates with the western blot data (to the left).
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Western blot analysis of FAP-1 using primary antibody dilution ratio of 1:2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
Specific Publications For Anti-PTPN13/PTPL1 (FAP1) Antibody (A02481)
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Customer Q&As
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5 Customer Q&As for Anti-PTPN13/PTPL1 (FAP1) Antibody
Question
Do you have a BSA free version of anti-PTPN13/PTPL1 (FAP1) antibody A02481 available?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PTPN13/PTPL1 (FAP1) antibody A02481 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-03
Question
Would A02481 anti-PTPN13/PTPL1 (FAP1) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
It shows on the product datasheet, A02481 anti-PTPN13/PTPL1 (FAP1) antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-26
Question
I have a question about product A02481, anti-PTPN13/PTPL1 (FAP1) antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02481 anti-PTPN13/PTPL1 (FAP1) antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-03
Question
We are currently using anti-PTPN13/PTPL1 (FAP1) antibody A02481 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
The anti-PTPN13/PTPL1 (FAP1) antibody (A02481) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-18
Question
Is a blocking peptide available for product anti-PTPN13/PTPL1 (FAP1) antibody (A02481)?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
We do provide the blocking peptide for product anti-PTPN13/PTPL1 (FAP1) antibody (A02481). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-18
