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Product Info Summary
| SKU: | A04617 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PTPRN2/Ptp Ia 2Beta Antibody
SKU/Catalog Number
A04617
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PTPRN2/Ptp Ia 2Beta Antibody (Catalog # A04617). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTPRN2/Ptp Ia 2Beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04617)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PTPRN2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04617 is reactive to PTPRN2 in Human, Mouse
Applications
A04617 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
111.271kDa
Background of PTPRN2
Implicated in development of nervous system and pancreatic endocrine cells.
Kawasaki E., Biochem. Biophys. Res. Commun. 227:440-447(1996).
Smith P.D., Biochem. Biophys. Res. Commun. 229:402-411(1996).
Cui L., J. Biol. Chem. 271:24817-24823(1996).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from NIH/3T3 cells, using PTPRN2 antibody A04617.
Protein Target Info & Infographic
Gene/Protein Information For PTPRN2 (Source: Uniprot.org, NCBI)
Gene Name
PTPRN2
Full Name
Receptor-type tyrosine-protein phosphatase N2
Weight
111.271kDa
Superfamily
protein-tyrosine phosphatase family
Alternative Names
EC 3.1.3.48; IA-2beta; IAR/receptor-like protein-tyrosine phosphatase; ICAARIARPTPRP; Islet cell autoantigen-related protein; KIAA0387; phogrin; protein tyrosine phosphatase receptor pi; protein tyrosine phosphatase, receptor type, N polypeptide 2; receptor-type tyrosine-protein phosphatase N2; R-PTP-N2; tyrosine phosphatase IA-2 beta PTPRN2 IA-2beta, IAR, ICAAR, PTPRP, R-PTP-N2 protein tyrosine phosphatase receptor type N2 receptor-type tyrosine-protein phosphatase N2|IAR/receptor-like protein-tyrosine phosphatase|islet cell autoantigen-related protein|phogrin|protein tyrosine phosphatase receptor pi|protein tyrosine phosphatase, receptor type, N polypeptide 2|tyrosine phosphatase IA-2 beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRN2, check out the PTPRN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTPRN2/Ptp Ia 2Beta Antibody (A04617)
Hello CJ!
No publications found for A04617
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-PTPRN2/Ptp Ia 2Beta Antibody
Question
I have attached the WB image, lot number and protocol we used for fetal brain using anti-PTPRN2/Ptp Ia 2Beta antibody A04617. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
Is a blocking peptide available for product anti-PTPRN2/Ptp Ia 2Beta antibody (A04617)?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We do provide the blocking peptide for product anti-PTPRN2/Ptp Ia 2Beta antibody (A04617). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-06
Question
I have a question about product A04617, anti-PTPRN2/Ptp Ia 2Beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04617 anti-PTPRN2/Ptp Ia 2Beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-21
Question
I see that the anti-PTPRN2/Ptp Ia 2Beta antibody A04617 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-15
Question
Is this A04617 anti-PTPRN2/Ptp Ia 2Beta antibody reactive to the isotypes of PTPRN2?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The immunogen of A04617 anti-PTPRN2/Ptp Ia 2Beta antibody is Synthesized peptide derived from internal of human PTPRN2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-PTPRN2/Ptp Ia 2Beta antibody A04617. Let me know if you need anything else.
K. Krishna
Verified customer
Asked: 2019-10-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
Does anti-PTPRN2/Ptp Ia 2Beta antibody A04617 work on canine WB with pancreas?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Our lab technicians have not validated anti-PTPRN2/Ptp Ia 2Beta antibody A04617 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PTPRN2/Ptp Ia 2Beta antibody A04617 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine pancreas in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-17
Question
Will anti-PTPRN2/Ptp Ia 2Beta antibody A04617 work for WB with fetal brain?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
According to the expression profile of fetal brain, PTPRN2 is highly expressed in fetal brain. So, it is likely that anti-PTPRN2/Ptp Ia 2Beta antibody A04617 will work for WB with fetal brain.
Boster Scientific Support
Answered: 2019-01-30
Question
Is there a BSA free version of anti-PTPRN2/Ptp Ia 2Beta antibody A04617 available?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PTPRN2/Ptp Ia 2Beta antibody A04617 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-12
Question
We are currently using anti-PTPRN2/Ptp Ia 2Beta antibody A04617 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
The anti-PTPRN2/Ptp Ia 2Beta antibody (A04617) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-25
Question
We are interested in to test anti-PTPRN2/Ptp Ia 2Beta antibody A04617 on mouse fetal brain for research purposes, then I may be interested in using anti-PTPRN2/Ptp Ia 2Beta antibody A04617 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Li
Verified customer
Asked: 2017-08-04
Answer
The products we sell, including anti-PTPRN2/Ptp Ia 2Beta antibody A04617, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-04
Question
I was wanting to use your anti-PTPRN2/Ptp Ia 2Beta antibody for WB for mouse fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse fetal brain identification?
M. Baker
Verified customer
Asked: 2016-09-05
Answer
You can see on the product datasheet, A04617 anti-PTPRN2/Ptp Ia 2Beta antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-05
Question
Would A04617 anti-PTPRN2/Ptp Ia 2Beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Wu
Verified customer
Asked: 2013-06-06
Answer
It shows on the product datasheet, A04617 anti-PTPRN2/Ptp Ia 2Beta antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-06-06
