Product Info Summary
SKU: | A02111-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Pumilio 1 PUM1 Antibody
SKU/Catalog Number
A02111-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Pumilio 1 PUM1 Antibody catalog # A02111-1. Tested in WB,ICC,IF,IHC,Flow Cytometry,IP applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pumilio 1 PUM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02111-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Shb around the phosphorylation site of Y246.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02111-1 is reactive to PUM1 in Human, Mouse
Applications
A02111-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
126.473kDa
Background of PUM1
Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2) By similarity. Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage. Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth.
Hateboer G., EMBO J. 14:3159-3169(1995).
Deloukas P., Nature 429:375-381(2004).
Matsuoka S., Science 316:1160-1166(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
IP: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of PUM1 using Anti-Pumilio 1 PUM1 Antibody (A02111-1).
ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Pumilio 1 PUM1 Antibody A02111-1
Western blot analysis of Pumilio 1 on SiHa cell using anti-Pumilio 1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For PUM1 (Source: Uniprot.org, NCBI)
Gene Name
PUM1
Full Name
Pumilio homolog 1
Weight
126.473kDa
Alternative Names
HSPUM; KIAA0099PUML1; PUM1; PUMH; PUMH1; PUMH1pumilio homolog 1; pumilio (Drosophila) homolog 1; pumilio homolog 1 (Drosophila); Pumilio-1; PUML1 PUM1 HSPUM, PUMH, PUMH1, PUML1, SCA47 pumilio RNA binding family member 1 pumilio homolog 1|pumilio-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PUM1, check out the PUM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PUM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pumilio 1 PUM1 Antibody (A02111-1)
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No publications found for A02111-1
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Customer Reviews
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