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Product Info Summary
| SKU: | M06224 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PURB Antibody (C-term)
SKU/Catalog Number
M06224
Size
50 µl
Description
Boster Bio Anti-PURB Antibody (C-term) (Catalog # M06224). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PURB Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06224)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2117CT1176.48.6
Isotype
IgG1,κ
Immunogen
This PURB antibody is generated from a mouse immunized with a recombiant protein of human PURB.
Reactive Species
M06224 is reactive to PURB in Human, Mouse
Calculated molecular weight
33.2 kDa
Background of PURB
Has capacity to bind repeated elements in single- stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine- rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06224 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
Validation Images & Assay Conditions
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All lanes : Anti-PURB Antibody (C-term) at 1:8000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: HeLa whole cell lysate
Lane 3: NCI-H460 whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: MCF-7 whole cell lysate
Lane 6: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-PURB Antibody (C-term) (M06224)
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