This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
LOC581356 antibody (DZ41739) performs well in IF, WB and shows reactivity with Purple sea urchin.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Western blot analysis of LOC581356 using anti-LOC581356 antibody (DZ41739).
Electrophoresis was performed on a 10% polyacrylamide gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Purple sea urchin embryo lysate lysates,
After electrophoresis, proteins were transferred to a PVDF membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA-TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC581356 antigen affinity purified polyclonal antibody (DZ41739) at 1:2000 with 1%BSA overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 2 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LOC581356 at approximately 84 kDa.
Electrophoresis was performed on a 10% polyacrylamide gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Purple sea urchin embryo lysate lysates,
After electrophoresis, proteins were transferred to a PVDF membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA-TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC581356 antigen affinity purified polyclonal antibody (DZ41739) at 1:2000 with 1%BSA overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 2 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LOC581356 at approximately 84 kDa.
Bundle Save
Anti-Purple sea urchin LOC581356 Antibody
LOC581356 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Purple sea urchin | Rabbit | IF, WB |
Recommend Dilution
Application
Dilution
Species
Western Blot
1:2000
Immunofluorescence
5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Purple sea urchin LOC581356 Antibody
View all LOC581356 Antibodies
View all LOC581356 Antibodies
Form
Liquid
Uniprot
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Cite This Product
Anti-Purple sea urchin LOC581356 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41739)
Review
Have you used Anti-Purple sea urchin LOC581356 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-Purple sea urchin LOC581356 Antibody
One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.
| SKU | DZ41739 |
|---|---|
| Application | Western Blot |
| Sample | egg lysate |
| Sample Processing Description | Egg lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis. |
| Primary Antibody | Anti-Purple sea urchin LOC581356 Antibody |
| Primary Incubation | 1:2000, incubated overnight at 4°C. |
| Secondary Antibody | Goat anti-rabbit IgG-HRP |
| Secondary Incubation | 2 hour at room temperature. |
| Detection | Chemiluminescent detection. |
| Results Summary | One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested. |
Mamiko Yajima
Verified customer
Submitted 2026-01-12
Product Promise
We promise Anti-Purple sea urchin LOC581356 Antibody performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
DZ41739
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026