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Product Info Summary
| SKU: | DZ41739 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Purple sea urchin |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Purple sea urchin LOC581356 Antibody
SKU/Catalog Number
DZ41739
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-Purple sea urchin LOC581356 Antibody catalog # DZ41739. This antibody reacts with Purple sea urchin.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-Purple sea urchin LOC581356 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41739)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Reactive Species
DZ41739 is reactive to LOC581356 in Purple sea urchin
Application & Images
Applications
DZ41739 is guaranteed for IF, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot | 1:2000 | |
| Immunofluorescence | 5 μg/ml |
Validation Images & Assay Conditions
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Western blot analysis of LOC581356 using anti-LOC581356 antibody (DZ41739).
Electrophoresis was performed on a 10% polyacrylamide gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Purple sea urchin embryo lysate lysates,
After electrophoresis, proteins were transferred to a PVDF membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA-TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC581356 antigen affinity purified polyclonal antibody (DZ41739) at 1:2000 with 1%BSA overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 2 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LOC581356 at approximately 84 kDa.
Specific Publications For Anti-Purple sea urchin LOC581356 Antibody (DZ41739)
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Customer Reviews
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1 Reviews For Anti-Purple sea urchin LOC581356 Antibody
One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested.
Excellent
| SKU | DZ41739 |
|---|---|
| Application | Western Blot |
| Sample | egg lysate |
| Sample Processing Description | Egg lysate was prepared and loaded onto a 10% polyacrylamide gel for electrophoresis. |
| Primary Antibody | Anti-Purple sea urchin LOC581356 Antibody |
| Primary Incubation | 1:2000, incubated overnight at 4°C. |
| Secondary Antibody | Goat anti-rabbit IgG-HRP |
| Secondary Incubation | 2 hour at room temperature. |
| Detection | Chemiluminescent detection. |
| Results Summary | One major band was detected at the expected size (84 kDa), while multiple minor bands were detected in the egg lysate under the condition tested. |
Mamiko Yajima
Verified customer
Submitted 2026-01-12
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