Anti-PXK Antibody Picoband™

Product Name

Anti-PXK Antibody Picoband™

View all PXK Antibodies

SKU/Catalog Number

A07555-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PXK Antibody Picoband™ catalog # A07555-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PXK Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07555-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PXK recombinant protein (Position: E5-K576).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07555-1 is reactive to PXK in Human, Monkey

Applications

A07555-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

64.95kDa

Background of PXK

PX domain containing serine/threonine kinase also known as PXK is a protein which in humans is encoded by the PXK gene. This gene encodes a phox (PX) domain-containing protein which may be involved in synaptic transmission and the ligand-induced internalization and degradation of epidermal growth factors. Variations in this gene may be associated with susceptibility to systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Monkey
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PXK using anti-PXK antibody (A07555-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PXK antigen affinity purified polyclonal antibody (Catalog # A07555-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PXK at approximately 65 kDa. The expected band size for PXK is at 65 kDa.

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Figure 2. IF analysis of PXK using anti-PXK antibody (A07555-1).
PXK was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PXK Antibody (A07555-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PXK (Source: Uniprot.org, NCBI)

Alternative Names

FLJ20335; K-ATPase long form; K-ATPase; modulator of Na; MONaKA; PX domain containing serine/threonine kinase; PX domain-containing protein kinase-like protein; PX ser/thr kinase v2; PX serine/threonine kinase PXK MONAKA, SLOB PX domain containing serine/threonine kinase like PX domain-containing protein kinase-like protein|PX ser/thr kinase v2|PX serine/threonine kinase|modulator of Na,K-ATPase long form

For more info on PXK, check out the PXK Infographic

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In this infographic, you will see the following information for PXK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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