Anti-R-spondin-1 RSPO1 Antibody

Product Name

Anti-R-spondin-1 RSPO1 Antibody

View all R-Spondin 1 Antibodies

SKU/Catalog Number

A02857-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-R-spondin-1 RSPO1 Antibody (Catalog # A02857-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

RSPO1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-R-spondin-1 RSPO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02857-1)

Host

Rabbit

Contents

RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

Anti-RSPO1 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.

Reactive Species

A02857-1 is reactive to Rspo1 in Human, Mouse

Applications

A02857-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.332kDa

Background of R-Spondin 1

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.5-2 μg/mL; IHC: 2.5-5 μg/mL; IF: 5 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Click image to see more details

Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: RSPO1, A02857-1 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Click image to see more details

Western Blot Validation with Recombinant Protein
Loading: 30 ng of human RSPO1 recombinant protein per lane.Antibodies: RSPO1, A02857-1 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL, Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Click image to see more details

Western Blot Validation in Human Spleen
Loading: 15 μg of lysates per lane. Antibodies: RSPO1, A02857-1 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Click image to see more details

Western Blot Validation in Human Heart
Loading: 15 μg of lysates per lane. Antibodies: RSPO1

Click image to see more details

Immunofluorescence Validation of RSPO1 in Human Spleen
Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling RSPO1 with A02857-1 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).

Click image to see more details

Immunohistochemistry Validation of RSPO1 in Human Heart
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-RSPO1 antibody (A02857-1) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Click image to see more details

Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (A02857-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Click image to see more details

Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (A02857-1) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Gene/Protein Information For Rspo1 (Source: Uniprot.org, NCBI)

Alternative Names

Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1 Rspo1|R, R-, R-spondin, Rspondin|R-spondin 1|R-spondin-1|R-spondin homolog|cristin-3|cysteine-rich and single thrombospondin domain-containing protein 3|mCristin-3|roof plate-specific spondin-1|thrombospondin type 1 domain containing gene Rspondin

For more info on Rspo1, check out the Rspo1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Rspo1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Rspo1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis