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Product Info Summary
| SKU: | A05387 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-R-spondin-3 RSPO3 Antibody
SKU/Catalog Number
A05387
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-R-spondin-3 RSPO3 Antibody (Catalog # A05387). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
RSPO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-R-spondin-3 RSPO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05387)
Host
Rabbit
Contents
RSPO3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RSPO3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RSPO3. The immunogen is located within amino acids 200 - 250 of RSPO3.
Cross-reactivity
RSPO3 antibody is human and mouse reactive. At least three isoforms of RSPO3 are known to exist; this antibody will detect all three.
Reactive Species
A05387 is reactive to RSPO3 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
30.9 kDa
Background of RSPO3
RSPO3 is a member of the R-spondin family and plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis (1,2). RSPO3 has been shown to be involved in regulating the balance between angioblast and blood cell specification during embryonic vasculogenesis and angiogenesis and may be involved in tumor development (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05387 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RSPO3 antibody can be used for detection of RSPO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RSPO3 in 293 cell lysate with RSPO3 antibody at 1 μg/ml.
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Immunocytochemistry of RSPO3 in 293 cells with RSPO3 antibody at 5 μg/mL.
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Immunofluorescence of RSPO3 in 293 cells with RSPO3 antibody at 20 μg/mL.
Specific Publications For Anti-R-spondin-3 RSPO3 Antibody (A05387)
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