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Product Info Summary
| SKU: | A14567 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Rab L2A Antibody
SKU/Catalog Number
A14567
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rab L2A Antibody catalog # A14567. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab L2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14567)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RABL2A. AA range:179-228
Reactive Species
A14567 is reactive to RABL2A in Human
Calculated molecular weight
26.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14567 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of COLO205 cells using Rab L2A Polyclonal Antibody diluted at 1:500
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Rab L2A Antibody (A14567)
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Customer Q&As
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7 Customer Q&As for Anti-Rab L2A Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right uterine tube using anti-Rab L2A antibody A14567. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
See below the WB image, lot number and protocol we used for right uterine tube using anti-Rab L2A antibody A14567. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-Rab L2A antibody A14567 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The anti-Rab L2A antibody (A14567) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-18
Question
Is there a BSA free version of anti-Rab L2A antibody A14567 available?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Rab L2A antibody A14567 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-02
Question
My question regarding product A14567, anti-Rab L2A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Evans
Verified customer
Asked: 2019-01-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14567 anti-Rab L2A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-02
Question
I see that the anti-Rab L2A antibody A14567 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-10
Question
Does A14567 anti-Rab L2A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Roberts
Verified customer
Asked: 2013-08-19
Answer
You can see on the product datasheet, A14567 anti-Rab L2A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-19
