This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
RAB11B antibody (M04526) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Flow Cytometry analysis of Caco-2 cells using anti-RAB11B antibody (M04526).
Overlay histogram showing Caco-2 cells stained with M04526 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB11B Antibody (M04526, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Caco-2 cells stained with M04526 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB11B Antibody (M04526, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-RAB11B Antibody Picoband® (monoclonal, 6C5)
RAB11B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, ICC, WB |
Tested Applications
RAB11B antibody was positively detected by WB in:
human HEK293 tissue, human Hela whole cell, human A549 whole cell, human placenta whole cell, human HepG2 whole cellhuman Caco-2 whole cellhuman THP-1 whole cellhuman Raji whole cell, rat brain tissue, rat lung whole cell, rat spleen whole cell, rat C6 whole cell, mouse brain whole cellmouse lung whole cellmouse spleen whole cellmouse Neuro-2a whole cellmouse RAW2467 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RAB11B antibody was positively detected by ICC/IF in:
MCF7 cell
RAB11B antibody was positively detected by FC in:
Caco-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
Human
Tested
Rat
Tested
Mouse
Tested
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Monkey
Predicted
Zebrafish
Predicted
Product Information
Product Name
Anti-RAB11B Antibody Picoband® (monoclonal, 6C5)
View all RAB11B Antibodies
View all RAB11B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6C5
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RAB11B, which shares 97.4% and 100% amino acid (aa) sequence identity with mouse and rat RAB11B, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
24 kDa
Calculated Molecular Weight
24.5 kDa
Background of RAB11B
Ras-related protein Rab-11B is a protein that in humans is encoded by the RAB11B gene. It is mapped to 19p13.2. The Ras superfamily of small GTP-binding proteins, which includes the Ras, Ral, Rho, Rap, and Rab families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways.
Cite This Product
Anti-RAB11B Antibody Picoband® (monoclonal, 6C5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04526)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
97%
High Homology
Zebrafish
92%
High Homology
Drosophila
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for RAB11B antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RAB11B antibody
Flow Cytometry (FC) protocol for RAB11B antibody
Publications
Application:
Species:
Zhang C,Chen Y,Sun S,Zhang Y,Wang L,Luo Z,Liu M,Dong L,Dong N,Wu Q. A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells. Elife.2020 Nov 2;9:e56059. doi:10.7554/eLife.56059.PMID:33136001;PMCID:PMC
Species: Human
PMID: 33136001
Review
Have you used Anti-RAB11B Antibody Picoband® (monoclonal, 6C5)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-RAB11B Antibody Picoband® (monoclonal, 6C5) performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M04526
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 29, 2026