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Product Info Summary
| SKU: | M05148 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-RAB5C Antibody
SKU/Catalog Number
M05148
Size
50 µl
Description
Boster Bio Anti-RAB5C Antibody (Catalog # M05148). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RAB5C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05148)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1616CT314.65.31
Isotype
IgG2b,k
Immunogen
This RAB5C antibody is generated from a mouse immunized with a recombinant protein of human RAB5C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05148 is reactive to RAB5C in Human
Applications
M05148 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
23.483kDa
Background of RAB5C
Protein transport. Probably involved in vesicular traffic (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-RAB5C Antibody at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: U-2OS whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 23 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2OS cells stained with M05148(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M05148, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For RAB5C (Source: Uniprot.org, NCBI)
Gene Name
RAB5C
Full Name
Ras-related protein Rab-5C
Weight
23.483kDa
Superfamily
small GTPase superfamily
Alternative Names
L1880; MGC117217; RAB5C, member of RAS oncogene family; RAB5C, member RAS oncogene family; RAB5CL; RAB5L; RABLMGC138857; ras-related protein Rab-5C RAB5C L1880L, RAB5L, RABL, RAB5C RAB5C, member RAS oncogene family ras-related protein Rab-5C|RAB5C, member of RAS oncogene family
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB5C, check out the RAB5C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB5C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB5C Antibody (M05148)
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No publications found for M05148
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