Product Info Summary
SKU: | A00347-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Rad50 (A708) Antibody
SKU/Catalog Number
A00347-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rad50 (A708) Antibody catalog # A00347-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rad50 (A708) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00347-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RAD50.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00347-1 is reactive to RAD50 in Human, Mouse, Rat
Applications
A00347-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
153.892kDa
Background of RAD50
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Dolganov G.M., Mol. Cell. Biol. 16:4832-4841(1996).
Kim K.K., Gene 235:59-67(1999).
Carney J.P., Cell 93:477-486(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Rad50 (A708) Antibody A00347-1
Western blot (WB) analysis of Rad50 (A708) polyclonal antibody at 1:500 dilutionLane1:786-O whole cell lysate(40ug)
Lane2:U-87MG whole cell lysate(40ug)
Lane3:SGC-7901 whole cell lysate(40ug)
Lane4:SK-OVCAR3 whole cell lysate(40ug)
Lane5:The Testis tissue lysate of Mouse(40ug)
Lane6:The Testis tissue lysate of Rat(40ug)
Lane7:C6 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For RAD50 (Source: Uniprot.org, NCBI)
Gene Name
RAD50
Full Name
DNA repair protein RAD50
Weight
153.892kDa
Superfamily
SMC family
Alternative Names
DNA repair protein RAD50; EC 3.6; EC 3.6.1.15; EC 3.6.3; EC 3.6.3.27; hRad50; NBSLD; RAD50 (S. cerevisiae) homolog; RAD50 homolog (S. cerevisiae); Rad50; RAD502; RAD50-2 RAD50 NBSLD2, hRad50, RAD50 RAD50 double strand break repair protein DNA repair protein RAD50|RAD50 homolog, double strand break repair protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAD50, check out the RAD50 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAD50: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rad50 (A708) Antibody (A00347-1)
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No publications found for A00347-1
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