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Product Info Summary
| SKU: | M00347 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Rad50 Monoclonal Antibody
SKU/Catalog Number
M00347
BM5243 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rad50 Monoclonal Antibody catalog # M00347. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rad50 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00347)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACCG-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rad50 Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A.
Reactive Species
M00347 is reactive to RAD50 in Human, Mouse, Rat
Observed Molecular Weight
154 kDa
Calculated molecular weight
153.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00347 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: Jurkat cell,
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Western blot analysis of Rad50 expression in Jurkat cell lysate;
Specific Publications For Anti-Rad50 Monoclonal Antibody (M00347)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Rad50 Monoclonal Antibody
Question
Is there a BSA free version of anti-Rad50 Monoclonal antibody M00347 available?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rad50 Monoclonal antibody M00347 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-31
Question
We are currently using anti-Rad50 Monoclonal antibody M00347 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The anti-Rad50 Monoclonal antibody (M00347) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Rad50 Monoclonal antibody M00347. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-09
