Product Info Summary
SKU: | M00088-2 |
---|---|
Size: | 50 µl |
Reactive Species: | Human |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-RAD51 Antibody
SKU/Catalog Number
M00088-2
Size
50 µl
Description
Boster Bio Anti-RAD51 Antibody (Catalog # M00088-2). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RAD51 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00088-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1690CT361.33.68
Isotype
IgG2b,k
Immunogen
This RAD51 antibody is generated from a mouse immunized with a recombinant protein between 1-339 amino acids from human RAD51.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00088-2 is reactive to RAD51 in Human
Applications
M00088-2 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.966kDa
Background of RAD51
Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-RAD51 Antibody at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: A431 whole cell lysate
Lane 5: Ramos whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 37 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For RAD51 (Source: Uniprot.org, NCBI)
Gene Name
RAD51
Full Name
DNA repair protein RAD51 homolog 1
Weight
36.966kDa
Superfamily
RecA family
Alternative Names
BRCC5; HRAD51; HsRad51; HsT16930; RAD51 (S. cerevisiae) homolog (E coli RecA homolog); RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog A; Rad51; RAD51ABRCA1/BRCA2-containing complex, subunit 5; RAD51L3; RecA, E. coli, homolog of; RECADNA repair protein RAD51 homolog 1; RecA-like protein; recombination protein A RAD51 BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2A, RECA, RAD51 RAD51 recombinase DNA repair protein RAD51 homolog 1|BRCA1/BRCA2-containing complex, subunit 5|RAD51 homolog A|RecA, E. coli, homolog of|RecA-like protein|recombination protein A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAD51, check out the RAD51 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAD51: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAD51 Antibody (M00088-2)
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No publications found for M00088-2
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