Product Info Summary
| SKU: | A06127 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Rag C RRAGC Antibody
SKU/Catalog Number
A06127
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rag C RRAGC Antibody catalog # A06127. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rag C RRAGC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06127)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RRAGC. AA range:302-351
Reactive Species
A06127 is reactive to RRAGC in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06127 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
IP 1:50-200
ELISA 1:2000-20000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HepG2, A549, and LOVO cells, using RRAGC Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Rag C RRAGC Antibody (A06127)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Rag C RRAGC Antibody
Question
We are currently using anti-Rag C antibody A06127 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
The anti-Rag C antibody (A06127) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-17
Question
Does A06127 anti-Rag C antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can see on the product datasheet, A06127 anti-Rag C antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-04
Question
Does anti-Rag C antibody A06127 work for WB with ovary {eco:0000312?
E. Jones
Verified customer
Asked: 2018-02-20
Answer
According to the expression profile of ovary {eco:0000312, RRAGC is highly expressed in ovary {eco:0000312. So, it is likely that anti-Rag C antibody A06127 will work for WB with ovary {eco:0000312.
Boster Scientific Support
Answered: 2018-02-20
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary {eco:0000312 using anti-Rag C antibody A06127. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-08

