Anti-Ran-binding protein 10 RANBP10 Antibody

Product Name

Anti-Ran-binding protein 10 RANBP10 Antibody

View all RANBP10 Antibodies

SKU/Catalog Number

A09936

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ran-binding protein 10 RANBP10 Antibody (Catalog # A09936). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

RANBP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Ran-binding protein 10 RANBP10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09936)

Host

Rabbit

Contents

RANBP10 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RANBP10 antibody was raised against a 15 amino acid synthetic peptide near the center of human RANBP10. The immunogen is located within amino acids 380 - 430 of RANBP10.

Reactive Species

A09936 is reactive to RANBP10 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

67.3 kDa

Background of RANBP10

The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09936 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RANBP10 antibody can be used for detection of RANBP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RANBP10

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