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Product Info Summary
| SKU: | A15427-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Ran BP-6 Antibody
SKU/Catalog Number
A15427-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Ran BP-6 Antibody catalog # A15427-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ran BP-6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15427-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RANBP6. AA range:800-849
Reactive Species
A15427-1 is reactive to RANBP6 in Human, Monkey, Mouse, Rat
Calculated molecular weight
124.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15427-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Ran BP-6 Polyclonal Antibody
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Western blot analysis of lysates from COS7, K562, and COLO cells, using RANBP6 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western Blot analysis of Various cells using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody was diluted at 1:10000 25°C, 1.5hours
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Ran BP-6 Antibody (A15427-1)
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5 Customer Q&As for Anti-Ran BP-6 Antibody
Question
We are currently using anti-Ran BP-6 antibody A15427-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The anti-Ran BP-6 antibody (A15427-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-27
Question
I was wanting to use your anti-Ran BP-6 antibody for WB for mouse endothelial cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse endothelial cell identification?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
It shows on the product datasheet, A15427-1 anti-Ran BP-6 antibody has been tested for WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse endothelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-12
Question
Is this A15427-1 anti-Ran BP-6 antibody reactive to the isotypes of RANBP6?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The immunogen of A15427-1 anti-Ran BP-6 antibody is Synthesized peptide derived from the Internal region of human Ran BP-6. at AA rangle: 770-850. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-24
Question
Is a blocking peptide available for product anti-Ran BP-6 antibody (A15427-1)?
R. Lewis
Verified customer
Asked: 2018-11-21
Answer
We do provide the blocking peptide for product anti-Ran BP-6 antibody (A15427-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-11-21
Question
Do you have a BSA free version of anti-Ran BP-6 antibody A15427-1 available?
G. Carter
Verified customer
Asked: 2014-02-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ran BP-6 antibody A15427-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-02-24
