Click image to see more details
Product Info Summary
| SKU: | A06527 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-RapGEF1/Grf2 Antibody
SKU/Catalog Number
A06527
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RapGEF1/Grf2 Antibody (Catalog# A06527). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RapGEF1/Grf2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06527)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RAPGEF1. Immunogen sequence location: 470-519
Reactive Species
A06527 is reactive to RAPGEF1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
120.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06527 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:5000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using RapGEF1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RapGEF1/Grf2 Antibody (A06527)
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Customer Q&As
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3 Customer Q&As for Anti-RapGEF1/Grf2 Antibody
Question
I was wanting to use your anti-RapGEF1/Grf2 antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
As indicated on the product datasheet, A06527 anti-RapGEF1/Grf2 antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-17
Question
Will anti-RapGEF1/Grf2 antibody A06527 work for IHC with leukemic t-cell?
L. Zhao
Verified customer
Asked: 2019-09-26
Answer
According to the expression profile of leukemic t-cell, RAPGEF1 is highly expressed in leukemic t-cell. So, it is likely that anti-RapGEF1/Grf2 antibody A06527 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-09-26
Question
Is this A06527 anti-RapGEF1/Grf2 antibody reactive to the isotypes of RAPGEF1?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The immunogen of A06527 anti-RapGEF1/Grf2 antibody is A peptide derived from human RAPGEF1. Immunogen sequence location: 470-519. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-21
