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Product Info Summary
| SKU: | M06527 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RAPGEF1 Antibody (Center)
SKU/Catalog Number
M06527
Size
80 µl
Description
Boster Bio Anti-RAPGEF1 Antibody (Center) (Catalog # M06527). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RAPGEF1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06527)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RAPGEF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 536-569 amino acids from the Central region of human RAPGEF1.
Reactive Species
M06527 is reactive to RAPGEF1 in Human
Calculated molecular weight
120.5 kDa
Background of RAPGEF1
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06527 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-RAPGEF1 Antibody (Center) at 1:1000-1:2000 dilution
Lane 1: K562 whole cell lysates
Lane 2: HepG2 whole cell lysates
Lane 3: MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 121 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-RAPGEF1 Antibody (Center)at 1:2000 dilution + Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 121 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-RAPGEF1 Antibody (Center) (M06527)
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