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Product Info Summary
| SKU: | A12923-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RAPGEF5 Antibody
SKU/Catalog Number
A12923-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RAPGEF5 Antibody (Catalog# A12923-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAPGEF5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12923-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RAPGEF5. Immunogen sequence location: 431 - 480
Reactive Species
A12923-1 is reactive to RAPGEF5 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
67.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12923-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of the lysates from COLO205 cells using RAPGEF5 antibody.
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Western blot analysis of lysates from RAW264.7 cells, using RAPGEF5 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RAPGEF5 Antibody (A12923-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-RAPGEF5 Antibody
Question
We are currently using anti-RAPGEF5 antibody A12923-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-RAPGEF5 antibody (A12923-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Do you have a BSA free version of anti-RAPGEF5 antibody A12923-1 available?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RAPGEF5 antibody A12923-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-15
Question
See below the WB image, lot number and protocol we used for brain using anti-RAPGEF5 antibody A12923-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-31
Question
I am interested in to test anti-RAPGEF5 antibody A12923-1 on human brain for research purposes, then I may be interested in using anti-RAPGEF5 antibody A12923-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Moore
Verified customer
Asked: 2014-07-21
Answer
The products we sell, including anti-RAPGEF5 antibody A12923-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-07-21
