Anti-RAPGEF5 Antibody Picoband™

Product Name

Anti-RAPGEF5 Antibody Picoband™

View all RAPGEF5 Antibodies

SKU/Catalog Number

A12923-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RAPGEF5 Antibody Picoband™ catalog # A12923-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RAPGEF5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12923-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human RAPGEF5 recombinant protein (Position: E164-H574). Human RAPGEF5 shares 95.6% and 96.4% amino acid (aa) sequence identity with mouse and rat RAPGEF5, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A12923-2 is reactive to RAPGEF5 in Human, Mouse, Rat

Applications

A12923-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

67.733kDa

Background of RAPGEF5

Rap guanine nucleotide exchange factor 5 is a protein that in humans is encoded by the RAPGEF5 gene. Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RAPGEF5 using anti-RAPGEF5 antibody (A12923-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAPGEF5 antigen affinity purified polyclonal antibody (Catalog # A12923-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAPGEF5 at approximately 75 kDa. The expected band size for RAPGEF5 is at 68 kDa.

Gene/Protein Information For RAPGEF5 (Source: Uniprot.org, NCBI)

Alternative Names

GFRREPAC; Guanine nucleotide exchange factor for Rap1; KIAA0277M-Ras-regulated GEF; M-Ras-regulated Rap GEF; MRGEF; MR-GEFrap guanine nucleotide exchange factor 5; Rap guanine nucleotide exchange factor (GEF) 5; Related to Epac; Repac RAPGEF5 GFR, MR-GEF, REPAC Rap guanine nucleotide exchange factor 5 rap guanine nucleotide exchange factor 5|M-Ras-regulated GEF|M-Ras-regulated Rap GEF|Rap guanine nucleotide exchange factor (GEF) 5|guanine nucleotide exchange factor for Rap1|related to Epac

For more info on RAPGEF5, check out the RAPGEF5 Infographic

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In this infographic, you will see the following information for RAPGEF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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