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Product Info Summary
| SKU: | A05683 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Ras-related protein Rab-21 RAB21 Antibody
SKU/Catalog Number
A05683
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ras-related protein Rab-21 RAB21 Antibody (Catalog # A05683). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RAB21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Ras-related protein Rab-21 RAB21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05683)
Host
Rabbit
Contents
RAB21 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
RAB21 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human RAB21. The immunogen is located within the last 50 amino acids of RAB21.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is predicted to not cross-react with other Ras-related proteins
Reactive Species
A05683 is reactive to RAB21 in Human, Mouse, Rat
Applications
A05683 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
24.348kDa
Background of RAB21
RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER)-like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α-tail of endocytosed integrin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RAB21 antibody can be used for detection of RAB21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RAB21 in mouse kidney tissue lysate with RAB21 antibody at 1 μg/mL.
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Immunofluorescence of RAB21 in human kidney tissue with RAB21 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RAB21 (Source: Uniprot.org, NCBI)
Gene Name
RAB21
Full Name
Ras-related protein Rab-21
Weight
24.348kDa
Superfamily
small GTPase superfamily
Alternative Names
KIAA0118ras-related protein Rab-21; RAB21, member RAS oncogene family RAB21 RAB21, member RAS oncogene family ras-related protein Rab-21|GTP-binding protein RAB21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB21, check out the RAB21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ras-related protein Rab-21 RAB21 Antibody (A05683)
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No publications found for A05683
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