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- Table of Contents
RASL12 antibody (A15197-1) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HepG2 cells using anti-RASL12 antibody (A15197-1).
Overlay histogram showing HepG2 cells stained with A15197-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RASL12 Antibody (A15197-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HepG2 cells stained with A15197-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RASL12 Antibody (A15197-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-RASL12 Antibody Picoband®
RASL12 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
RASL12 antibody was positively detected by WB in:
human Caco-2 whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RASL12 antibody was positively detected by ICC/IF in:
A549 cell
RASL12 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Dog
Predicted
Predicted
Rat
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Mouse
Predicted
Predicted
Product Information
Product Name
Anti-RASL12 Antibody Picoband®
View all RASL12 Antibodies
View all RASL12 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human RASL12 recombinant protein (Position: K7-K261). Human RASL12 shares 93.3% amino acid (aa) sequence identity with mouse RASL12.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
29.7 kDa
Background of RASL12
Belonging to the small GTPase superfamily/Ras family, RASL12 is localized in the cellular membrane and cytoplasm. RASL12 has many important molecular functions including GTP binding, GTPase activity and nucleotide binding. The main biological function of this gene is to participate in the GTP catabolic process, signal transduction and small GTPase mediated signal transduction. RASL12 interacts with ACVR1, SMAD1, SMAD2, SMAD3 and SMURF2. Documented diseases associated with RASL12 include acute kidney tubular necrosis, intraepithelial neoplasm, retinitis, endometrial cancer, coronary artery disease and Huntington's disease.
Cite This Product
Anti-RASL12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15197-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
95%
High Homology
Dog
94%
High Homology
Rat
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Mouse
93%
High Homology
Zebrafish
64%
Low homology
Drosophila
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for RASL12 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RASL12 antibody
Flow Cytometry (FC) protocol for RASL12 antibody
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Product Promise
We promise Anti-RASL12 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A15197-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026