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Product Info Summary
| SKU: | A10330 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RBM16 SCAF8 Antibody
View all SCAF8/RBM16 Antibodies
SKU/Catalog Number
A10330
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RBM16 SCAF8 Antibody catalog # A10330. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RBM16 SCAF8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10330)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2F1
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human RBM16. at AA rangle: 550-630
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10330 is reactive to SCAF8 in Human, Mouse, Rat
Applications
A10330 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
140.519kDa
Background of SCAF8/RBM16
Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines. Anti-Cybr Antibody is suitable for researchers interested in the Research Areas of Chromatin, Immunology, Neuroscience, and Signal Transduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of JK cells using RBM16 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For SCAF8 (Source: Uniprot.org, NCBI)
Gene Name
SCAF8
Full Name
SR-related and CTD-associated factor 8
Weight
140.519kDa
Alternative Names
CCAP7; CDC5L complex-associated protein 7; KIAA1116RNA-binding protein 16; putative RNA-binding protein 16; RBM16; RNA binding motif protein 16; RNA-binding motif protein 16; SR-like CTD-associated factor 8; SR-related and CTD-associated factor 8; SR-related CTD-associated factor 8 SCAF8 RBM16 SR-related CTD associated factor 8 SR-related and CTD-associated factor 8|CDC5L complex-associated protein 7|RNA-binding motif protein 16|SR-like CTD-associated factor 8|protein SCAF8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCAF8, check out the SCAF8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCAF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RBM16 SCAF8 Antibody (A10330)
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No publications found for A10330
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-RBM16 SCAF8 Antibody
Question
Can you help my question with product A10330, anti-RBM16 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10330 anti-RBM16 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-16
Question
We are currently using anti-RBM16 antibody A10330 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The anti-RBM16 antibody (A10330) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-19
Question
Will anti-RBM16 antibody A10330 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
According to the expression profile of testis, SCAF8 is highly expressed in testis. So, it is likely that anti-RBM16 antibody A10330 will work for WB with testis.
Boster Scientific Support
Answered: 2019-07-11
Question
Does A10330 anti-RBM16 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
It shows on the product datasheet, A10330 anti-RBM16 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-04
